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KMID : 0382319890090010013
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Environmental Mutagens and Carcinogens
1989 Volume.9 No. 1 p.13 ~ p.22
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Environmental Toxic Agents on Genetic Material and Cellular Activity IV. Novobiocin-Mediated Inhibition of DNA Repair Synthesis in Synchronized Chinese Hamster Ovary Cells
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엄경일
김춘광/이천복/신은주/문용석
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Abstract
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The effect of novobiocin (NOV), and inhibitor of topoisomerase II, on ethyl methanesulfonate (EMS)-or bleomycin (BLM)-induced DNA repair synthesis was examined during the cell cycle of Chinese hamster ovary (CHO)-K1 cells. Three assays were employed in this study: cell survival, alkaline elution and unscheduled DNA synthesis. EMS was effective at killing CHO cells in G1 phase, wheras BLM preferentially killed cells in G2 and S phases. EMS induced the much more amount of DNA damage in G1 phase, while BLM induced in G2 phase than the other phases. The both of pre- and post-treatment with BOV inhibitied EMS- or BLM-induced DNA repair synthesis in G1 and G2 phases, and pretreatment with NOV inhibited more effectively than the post-treated group.
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KEYWORD
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